FRET and FLIM Techniques

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  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier
  • Pages : 560 pages
  • ISBN : 9780080915128
  • Rating : /5 from reviews
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Download or Read online FRET and FLIM Techniques full in PDF, ePub and kindle. this book written by Theodorus W. J. Gadella and published by Elsevier which was released on 29 July 2011 with total page 560 pages. We cannot guarantee that FRET and FLIM Techniques book is available in the library, click Get Book button and read full online book in your kindle, tablet, IPAD, PC or mobile whenever and wherever You Like. This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

FRET and FLIM Techniques

FRET and FLIM Techniques
  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier
  • Release : 29 July 2011
GET THIS BOOK FRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

FRET and FLIM Techniques

FRET and FLIM Techniques
  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier Science
  • Release : 05 January 2009
GET THIS BOOK FRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Molecular Imaging

Molecular Imaging
  • Author : Ammasi Periasamy,Richard Day
  • Publisher : Elsevier
  • Release : 28 April 2011
GET THIS BOOK Molecular Imaging

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and

FRET and FLIM Techniques

FRET and FLIM Techniques
  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier Science
  • Release : 05 January 2009
GET THIS BOOK FRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Fluorescence Spectroscopy and Microscopy

Fluorescence Spectroscopy and Microscopy
  • Author : Yves Engelborghs,Antonie J.W.G. Visser
  • Publisher : Humana Press
  • Release : 23 August 2016
GET THIS BOOK Fluorescence Spectroscopy and Microscopy

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written

FLIM Microscopy in Biology and Medicine

FLIM Microscopy in Biology and Medicine
  • Author : Ammasi Periasamy,Robert M. Clegg
  • Publisher : CRC Press
  • Release : 06 July 2009
GET THIS BOOK FLIM Microscopy in Biology and Medicine

Detecting Signals at the Single Molecule Level: Pioneering Achievements in Microscopy Recent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy’s (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional benefit of independence from fluorophore concentration and excitation intensity. Moreover, its unique sensitivity makes it an excellent reporter of conformational changes and of variations in the molecular surroundings of biological

Methods in Cellular Imaging

Methods in Cellular Imaging
  • Author : Ammasi Periasamy
  • Publisher : Springer
  • Release : 27 May 2013
GET THIS BOOK Methods in Cellular Imaging

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton

Advanced Photon Counting

Advanced Photon Counting
  • Author : Peter Kapusta,Michael Wahl,Rainer Erdmann
  • Publisher : Springer
  • Release : 23 April 2015
GET THIS BOOK Advanced Photon Counting

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Fö

Multiphoton Microscopy and Fluorescence Lifetime Imaging

Multiphoton Microscopy and Fluorescence Lifetime Imaging
  • Author : Karsten König
  • Publisher : Walter de Gruyter GmbH & Co KG
  • Release : 22 January 2018
GET THIS BOOK Multiphoton Microscopy and Fluorescence Lifetime Imaging

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence

Fluorescence Lifetime Spectroscopy and Imaging

Fluorescence Lifetime Spectroscopy and Imaging
  • Author : Laura Marcu,Paul M. W. French,Daniel S. Elson
  • Publisher : CRC Press
  • Release : 17 July 2014
GET THIS BOOK Fluorescence Lifetime Spectroscopy and Imaging

During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides

Methods in Cell Biology

Methods in Cell Biology
  • Author : Anonim
  • Publisher : Academic Press
  • Release : 16 May 2020
GET THIS BOOK Methods in Cell Biology

Methods in Cell Biology, Volume 158, the latest release in this series, highlights new advances in the field, with this release covering How to orient cells in micro-cavities for high resolution imaging of cytokinesis and lumen formation, A body-on-a-chip (BOC) system for studying gut-liver interaction, Manipulating cultured mammalian cells for mitosis research, Live-cell FLIM-FRET using a commercially available system, A comparative analysis of methods to measure kinetochore-microtubule attachment stability, A workflow for visualizing human cancer biopsies using large-format electron microscopy, Isolation

Understanding Light Microscopy

Understanding Light Microscopy
  • Author : Jeremy Sanderson
  • Publisher : Wiley
  • Release : 28 May 2019
GET THIS BOOK Understanding Light Microscopy

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and

Fluorescent Methods to Study Biological Membranes

Fluorescent Methods to Study Biological Membranes
  • Author : Yves Mely,Guy Duportail
  • Publisher : Springer Science & Business Media
  • Release : 10 October 2012
GET THIS BOOK Fluorescent Methods to Study Biological Membranes

Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting