Genome Editing

• Author : Kiran Musunuru
• Publisher : Academic Press
• Release : 05 March 2021

Genome Editing: A Practical Guide to Research and Clinical Applications is geared towards investigators interested in learning how to use CRISPR-Cas9-based technologies, with a focus on cardiovascular research and clinical applications. Covering a range of topics from the basics of genome editing to design considerations, to assessments and applications, this reference allows readers to get started and establish a full workflow from the beginning of the project to its full completion. With worked examples drawn from real-life experiments, as

• Author : Derek Tilghman Peters
• Publisher : Unknown
• Release : 18 January 2022

The properties of human pluripotent stem cells (hPSCs) make them uniquely suited to address this challenge - they retain a normal human genome in culture, can be genetically modified, and can be differentiated into multiple cell types. The work presented in this thesis demonstrates the use of hPSCs and genome editing technology to generate human disease models in vitro. We developed a genome editing system optimized for hPSCs that can be used to efficiently generate hPSC lines with targeted genetic

• Author : Kursad Turksen
• Publisher : Springer
• Release : 09 August 2016

This timely volume explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others. The final chapters round out the book with a discussion of

• Author : Jeff Hsu
• Publisher : Unknown
• Release : 18 January 2022

Coronary artery disease (CAD) and atrial fibrillation (AF) are two common complex diseases of the heart that both have strong genetic components. Although genome wide association studies in humans and genetic mapping studies in mouse models have identified genomic loci associated with these diseases, the functional genetic variants and the mechanisms by which they alter disease susceptibility are largely unknown. In this thesis we use mouse models and human atrial tissues to perform functional genomic studies to gain insight into

• Author : Giuseppe Limongelli
• Publisher : Elsevier Health Sciences
• Release : 02 April 2018

This issue of Heart Failure Clinics--edited by Drs. Giuseppe Limongelli, Sharlene Day, and Perry Elliott--will cover Clinical and Molecular Aspects of Cardiomyopathies: On the Road from Gene to Therapy. Topics include, but are not limited to, Epidemiology and Clinical aspects of Genetic Cardiomyopathies, Existing and New therapies, Gene editing and gene-based therapeutics, Controversies surrounding exercise in genetic cardiomyopathies, Genetic infiltrative cardiomyopathies, LV Noncompaction, Clinical presentation and natural history of hypertrophic cardiomyopathy in Rasopathies, and Moelcular basis and new treatments of

• Author : Xi Cheng,Bina Joe
• Publisher : Biota Publishing
• Release : 01 August 2015

Hypertension, or elevated blood pressure, is a major risk factor for various cardiovascular, renal diseases, and stroke. The form of hypertension with no identifiable cause is referred to as Essential Hypertension. Familial studies indicate that Essential Hypertension is heritable and, thereby, classical genetic approaches have been applied on both human and other mammalian models of hypertension to map the locations of the allelic variants within quantitative trait loci for blood pressure. The post genome era has further elevated this area

• Author : Annabelle Rodriguez-Oquendo
• Publisher : Academic Press
• Release : 07 September 2015

Translational Cardiometabolic Genomic Medicine, edited by Dr. Annabelle Rodriguez-Oquendo, is an important resource to postgraduate (medical, dental and graduate) students, postdoctoral fellows, basic scientists, and physician scientists seeking to understand and expand their knowledge base in the field of genomic medicine as it is applied to cardiometabolic diseases. This handbook integrates cutting-edge experimental approaches such as chromatin immunoprecipitation paired end tagging (CHIA-PET), to population studies such as the Multi-Ethnic Study of Atherosclerosis. It encompasses a range of book chapters that

• Author : Anonim
• Publisher : Academic Press
• Release : 12 June 2021

Reprogramming the Genome: CRISPR-Cas-based Human Disease Therapy, presents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 is a key technology for targeted genome editing and regulation in a number of organisms including mammalian cells. It is a rapid, simple, and cost-effective solution. CRISPR-Cas system has recently gained much scientific and public attention. This volume covers CRISPR-Cas9 based mammalian genome editing, creating disease models, cancer therapy, neurological, heredity, blood disorders, defective gene correction, stem cells therapy, epigenetic modifications, patents,

• Author : Krishnarao Appasani
• Publisher : Cambridge University Press
• Release : 30 June 2018

Recent advances in genome editing tools using endonucleases such as TALENs, ZFNs, and CRISPRs, combined with genomic engineering technologies, have opened up a wide range of opportunities from applications in the basic sciences and disease biology research, to the potential for clinical applications and the development of new diagnostic tools. This complete guide to endonuclease-based genomic engineering gives readers a thorough understanding of this rapidly expanding field. Chapters cover the discovery, basic science, and application of these techniques, focusing particularly

• Author : Krishnarao Appasani
• Publisher : Cambridge University Press
• Release : 31 July 2018

A complete guide to endonuclease-based genomic engineering, from basic science to application in disease biology and clinical treatment.

• Author : Jianyi Zhang,Vahid Serpooshan,James J. H. Chong,Philippe Menasché
• Publisher : Frontiers Media SA
• Release : 04 October 2021

• Author : Xiaojun Liu
• Publisher : BoD – Books on Demand
• Release : 05 September 2018

Genetics and genomics in poultry have been the most rapidly advancing subjects since the completion of the chicken genome sequence in 2004 and have been extensively used to understand the genetic determinants of complex traits. This book intends to provide readers with a comprehensive overview of the current progress in the application of genetic and genomic science in the poultry field. The contents cover genetic variation detection, selection methods for breeding, transgenesis and genome editing, genetic basis of disease resistance, control

• Author : Dr. Hakim Saboowala
• Publisher : Dr.Hakim Saboowala
• Release : 19 August 2020

A Glimpse of Recent/Current “Multidisciplinary Medical News.”, Due to an overwhelming response in the past, from the medicos abroad, I’m inspired to compile as usual thisupdated informative as well as interesting E- Medical Booklet to encourage the researches in various field of medicine! Thus, an effort has been made in this E-Booklet to include the following interesting cum informative Topics along with the relevant Images for the enthusiastic Medicos to access at one Click! 1. Hydrogen Sulphide can reverse

• Author : Yamin Liu
• Publisher : Unknown
• Release : 18 January 2022

Hypertrophic cardiomyopathy (HCM) is a common genetic cardiovascular disease present in 1 in 500 of the general population, leading to the most frequent cause of sudden death in young people (including trained athletes), heart failure, and stroke. HCM is an autosomal dominant inheritance, which is associated with a large number of mutations in genes encoding proteins of the cardiac sarcomere. Over the last 20 years, the recognition, diagnosis, and treatment of HCM have been improved dramatically. And moreover, recent advancement in genomic medicine,

• Author : Jeanette Erdmann,Alessandra Moretti
• Publisher : Springer Nature
• Release : 27 November 2019

This book provides a comprehensive summary of the latest developments in the field of the genomics of cardiac disease. Written and edited by leading clinicians and scientists involved in the analysis and therapy of genetic cardiac disorders, it discusses the genetic causes of a variety of cardiac diseases, such as the complex genetics and etiology of congenital heart diseases. It also explores sex differences in prevalent diseases, genetics-based therapeutic strategies and the use of various animal models and alternatives. The