Mass Spectrometry in Biophysics

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  • Author : Igor A. Kaltashov
  • Publisher : John Wiley & Sons
  • Pages : 320 pages
  • ISBN : 0471705160
  • Rating : /5 from reviews
CLICK HERE TO GET THIS BOOK >>>Mass Spectrometry in Biophysics

Download or Read online Mass Spectrometry in Biophysics full in PDF, ePub and kindle. this book written by Igor A. Kaltashov and published by John Wiley & Sons which was released on 06 May 2005 with total page 320 pages. We cannot guarantee that Mass Spectrometry in Biophysics book is available in the library, click Get Book button and read full online book in your kindle, tablet, IPAD, PC or mobile whenever and wherever You Like. The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical background and technical aspects of eachmethod, this much-needed reference offers an unparalleled overviewof the current state of biophysical MS. Mass Spectrometry inBiophysics begins with a helpful discussion of general biophysicalconcepts and MS-related techniques. Subsequent chaptersaddress: * Modern spectrometric hardware * High-order structure and dynamics as probed by various MS-basedmethods * Techniques used to study structure and behavior of non-nativeprotein states that become populated under denaturingconditions * Kinetic aspects of protein folding and enzyme catalysis * MS-based methods used to extract quantitative information onprotein-ligand interactions * Relation of MS-based techniques to other experimental tools * Biomolecular properties in the gas phase Fully referenced and containing a helpful appendix on the physicsof electrospray mass spectrometry, Mass Spectrometry in Biophysicsalso offers a compelling look at the current challenges facingbiomolecular MS and the potential applications that will likelyshape its future.

Mass Spectrometry in Biophysics

Mass Spectrometry in Biophysics
  • Author : Igor A. Kaltashov,Stephen J. Eyles
  • Publisher : John Wiley & Sons
  • Release : 06 May 2005
GET THIS BOOK Mass Spectrometry in Biophysics

The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical

Mass Spectrometry in Structural Biology and Biophysics

Mass Spectrometry in Structural Biology and Biophysics
  • Author : Igor A. Kaltashov,Stephen J. Eyles
  • Publisher : John Wiley & Sons
  • Release : 03 April 2012
GET THIS BOOK Mass Spectrometry in Structural Biology and Biophysics

With its detailed and systematic coverage of the current state of biophysical mass spectrometry (MS), here is one of the first systematic presentations of the full experimental array of MS-based techniques used in biophysics, covering both fundamental and practical issues. The book presents a discussion of general biophysical concepts and a brief overview of traditional biophysical techniques before outlining the more advanced concepts of mass spectrometry. The new edition gives an up-to-date and expanded coverage of experimental methodologies and a

Methods in Modern Biophysics

Methods in Modern Biophysics
  • Author : Bengt Nölting
  • Publisher : Springer Science & Business Media
  • Release : 09 March 2013
GET THIS BOOK Methods in Modern Biophysics

Incorporating dramatic recent advances, "Methods in Modern Biophysics" presents a fresh and timely introduction to modern biophysical methods. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, "Methods in Modern Biophysics" employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for

Development and Application of Mass Spectrometry based Biophysical Approaches

Development and Application of Mass Spectrometry based Biophysical Approaches
  • Author : Ying Zhang
  • Publisher : Unknown
  • Release : 08 December 2021
GET THIS BOOK Development and Application of Mass Spectrometry based Biophysical Approaches

Mass spectrometry (MS)-based biophysical approaches are new "tools" for protein characterization owing to its capability to analyze proteins and protein complexes that range in molecular weight from kDa to MDa. Protein characterization requires more than identifying the primary structure. More importantly, protein high order structures (i.e., secondary, tertiary and quaternary structures) are needed for biological studies. MS has become the major tool in studies of protein primary structure and post translational modifications (PTMs) over the past two decades.

Biophysical Mass Spectrometry Techniques for Probing the Higher Order Structure of Proteins and Complexes

Biophysical Mass Spectrometry Techniques for Probing the Higher Order Structure of Proteins and Complexes
  • Author : Sterling, Jr. (Harry John)
  • Publisher : Unknown
  • Release : 08 December 2021
GET THIS BOOK Biophysical Mass Spectrometry Techniques for Probing the Higher Order Structure of Proteins and Complexes

Electrospray ionization mass spectrometry (ESI-MS) is a powerful analytical platform for answering a wide variety of questions about the identity, quantity, structure, function, dynamics and energetics of biological molecules. Key advantages of ESI-MS include unrivaled specificity, attomole sensitivity, and the capacity for simultaneous analysis of complex mixtures with analyte masses that differ by less than 1 ppm. The low flow rates and sub-micron sized droplets formed with "nano" ESI allows biomolecular ions to be readily formed from purely aqueous or buffered

Molecular Biophysics for the Life Sciences

Molecular Biophysics for the Life Sciences
  • Author : Norma Allewell,Linda O. Narhi,Ivan Rayment
  • Publisher : Springer Science & Business Media
  • Release : 28 September 2013
GET THIS BOOK Molecular Biophysics for the Life Sciences

This volume provides an overview of the development and scope of molecular biophysics and in-depth discussions of the major experimental methods that enable biological macromolecules to be studied at atomic resolution. It also reviews the physical chemical concepts that are needed to interpret the experimental results and to understand how the structure, dynamics, and physical properties of biological macromolecules enable them to perform their biological functions. Reviews of research on three disparate biomolecular machines—DNA helicases, ATP synthases, and myosin--illustrate

Biophysical Characterization of Proteins in Developing Biopharmaceuticals

Biophysical Characterization of Proteins in Developing Biopharmaceuticals
  • Author : Damian J. Houde,Steven A. Berkowitz
  • Publisher : Elsevier
  • Release : 13 November 2019
GET THIS BOOK Biophysical Characterization of Proteins in Developing Biopharmaceuticals

Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today’s industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been

Biophysical Chemistry

Biophysical Chemistry
  • Author : Alan Cooper
  • Publisher : Royal Society of Chemistry
  • Release : 09 November 2015
GET THIS BOOK Biophysical Chemistry

Biophysical Chemistry covers the physical chemistry of biological macromolecules and the experimental techniques used to study them. Topics covered include: an introduction to biological molecules; spectroscopy, mass spectrometry and hydrodynamics of macromolecules; a ""bluffer's guide"" to molecular thermodynamics; biomolecular kinetics; chromatography and electrophoresis; and single-molecule methods. The easily digestible, pragmatic approach captures the reader with the fascinating challenges the subject poses for theoretical and experimental scientists. This book will be ideal for early undergraduates studying chemical or physical sciences and

Hydrogen Exchange Mass Spectrometry of Proteins

Hydrogen Exchange Mass Spectrometry of Proteins
  • Author : David D. Weis
  • Publisher : John Wiley & Sons
  • Release : 21 March 2016
GET THIS BOOK Hydrogen Exchange Mass Spectrometry of Proteins

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the

Introduction to Biophysical Methods for Protein and Nucleic Acid Research

Introduction to Biophysical Methods for Protein and Nucleic Acid Research
  • Author : Jay A. Glasel,Murray P. Deutscher
  • Publisher : Academic Press
  • Release : 20 November 1995
GET THIS BOOK Introduction to Biophysical Methods for Protein and Nucleic Acid Research

The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment. Key Features * Modern Use of Mass Spectroscopy * NMR Spectroscopy * Molecular

Mass Spectrometry based Strategies for Protein Biophysics

Mass Spectrometry based Strategies for Protein Biophysics
  • Author : Richard Yu-Cheng Huang
  • Publisher : Unknown
  • Release : 08 December 2021
GET THIS BOOK Mass Spectrometry based Strategies for Protein Biophysics

Two important biophysical characteristics of proteins, their interaction with ligands and their post-translational modifications, modulate various biological processes including signal transduction, chemical synthesis, and cell function. Protein-ligand interactions include the interactions with protein, peptide, DNA, and metal. Characterization of the physical properties of these interactions (binding interfaces, binding affinities, and the protein conformational changes due to the binding) is essential in understanding the mechanism of related diseases and, more importantly, in future drug design. Mass spectrometry, with its own revolution

Novel Mass Spectrometry based Strategies for Biophysical Analysis of Proteins and Protein ligand Complexes

Novel Mass Spectrometry based Strategies for Biophysical Analysis of Proteins and Protein ligand Complexes
  • Author : Liangjie Tang
  • Publisher : ProQuest
  • Release : 08 December 2021
GET THIS BOOK Novel Mass Spectrometry based Strategies for Biophysical Analysis of Proteins and Protein ligand Complexes

The third protocol developed here is a SUPREX-like protocol that employs a chemical modification reaction (i.e., oxidation reaction) in place of the amide H/D exchange reaction in SUPREX. This SUPREX-like protocol, termed SPROX (Stability of Proteins from Rates of Oxidation) was developed and tested to detect and quantify protein-ligand binding using cyclophilin A and BCAII as model systems.

Biophysical Techniques in Photosynthesis

Biophysical Techniques in Photosynthesis
  • Author : Thijs Aartsma,Jörg Matysik
  • Publisher : Springer Science & Business Media
  • Release : 01 February 2008
GET THIS BOOK Biophysical Techniques in Photosynthesis

Since the first volume on Biophysical Techniques in Photosynthesis Research, published in 1996, new experimental techniques and methods have been devised at a rapid pace. The present book is a sequel which complements the publication of the first volume by providing a comprehensive overview of the most important new techniques developed over the past ten years, especially those that are relevant for research on the mechanism and fundamental aspects of photosynthesis.